kristian helin  (Addgene inc)

Journal: Journal of Cellular and Molecular Medicine

Article Title: Classification of Paediatric Celiac Disease Using RNA Sequencing and Real‐Time PCR of Duodenal Biomarkers

doi: 10.1111/jcmm.70854

Figure Lengend Snippet: (a–d) Expression of (a) CYP3A4 , (b) LCT , (c) CXCL11 , and (d) E2F5 , normalised to the lowest expression in the whole dataset and presented on a log 2 scale. The grey marker in group BiopsyDiag represents the misclassified biopsy from case 51, and the grey marker in group TGdiag represents the misclassified biopsy from case 55. From left to right in each plot, the groups are: Not CD, Normalised CD, Potential CD without progression, Potential CD with progression, Potential CD with high anti‐TG2 and progression, Biopsy‐based CD diagnosis, Anti‐TG2 based CD diagnosis, and Marsh 3 histopathology and negative anti‐TG2.

Article Snippet: E2F5 , E2F transcription factor 5 , 8q21.2 , Hs00231092_m1.

Techniques: Expressing, Marker, Biomarker Discovery, Histopathology

Journal: Journal of Translational Medicine

Article Title: Exosomes loaded with the anti-cancer molecule mir-1-3p inhibit intrapulmonary colonization and growth of human esophageal squamous carcinoma cells

doi: 10.1186/s12967-024-05997-9

Figure Lengend Snippet: miR-1-3p directly targets and down-regulates E2F5 expression. The differentially expressed genes in the Exo-miR-1-3p group compared with the Exo-NC group, shown in the volcano plot ( A ), and the KEGG pathway analysis of differentially expressed genes ( B ). ( C ) Predicted target genes of miR-1-3p with TargetScan and miRWalk were cross-analyzed with significantly expression down-regulated genes. ( D ) E2F5 mRNA level after Exo-miR-1-3p incubation. ( E ) Putative binding sequence of miR-1-3p to the E2F5 mRNA 3’UTR and results of dual luciferase reporter assays. E2F5 protein expression of KYSE150 and Eca109 cells transfected with miR-1-3p mimics ( F ) or miR-1-3p inhibitor ( G ). Immunohistochemical staining ( H ) and H-scores ( I ) of E2F5 in mouse lung tissues. * P < 0.05, *** P < 0.001

Article Snippet: Antibodies information used in this study is provided below: anti-TSG101 (1:2000, 14497-1-AP, Proteintech), anti-CD81 (66866-1-Ig), anti-CD9 (60232-1-Ig), anti-E2F5 (1:2000, bs-1734R, Bioss), anti-GAPDH (1:5000, 60004-1-Ig, Proteintech), anti-p-p38 (1:1000, 4511, CST), anti-p38 (1:5000, 66234-1-lg, Proteintech), anti-p-ERK (1:1000, AP0974, ABclonal), anti-ERK (1:700, A4782, ABclonal), anti-p-Akt (1:2000, 28731-1-AP, Proteintech), anti-p-mTOR (1:500, AF3308, Affinity), HRP Goat anti-Rabbit IgG (1: 5000, AS014, ABclonal), HRP Goat anti-Mouse IgG (1: 5000, AS003, ABclonal).

Techniques: Expressing, Incubation, Binding Assay, Sequencing, Luciferase, Transfection, Immunohistochemical staining, Staining

Journal: Journal of Translational Medicine

Article Title: Exosomes loaded with the anti-cancer molecule mir-1-3p inhibit intrapulmonary colonization and growth of human esophageal squamous carcinoma cells

doi: 10.1186/s12967-024-05997-9

Figure Lengend Snippet: miR-1-3p inhibits proliferation and migration of ESCC cells in vitro by down-regulating E2F5. The miR-1-3p levels ( A ), E2F5 mRNA levels ( B ), and E2F5 protein levels in KYSE150 and Eca109 cells in vitro co-transfected with miR-1-3p mimics and E2F5 expression plasmids. The proliferation ( D ) and migration ( E and F ) of transfected cells. ns, no significance; * P < 0.05, *** P < 0.001

Article Snippet: Antibodies information used in this study is provided below: anti-TSG101 (1:2000, 14497-1-AP, Proteintech), anti-CD81 (66866-1-Ig), anti-CD9 (60232-1-Ig), anti-E2F5 (1:2000, bs-1734R, Bioss), anti-GAPDH (1:5000, 60004-1-Ig, Proteintech), anti-p-p38 (1:1000, 4511, CST), anti-p38 (1:5000, 66234-1-lg, Proteintech), anti-p-ERK (1:1000, AP0974, ABclonal), anti-ERK (1:700, A4782, ABclonal), anti-p-Akt (1:2000, 28731-1-AP, Proteintech), anti-p-mTOR (1:500, AF3308, Affinity), HRP Goat anti-Rabbit IgG (1: 5000, AS014, ABclonal), HRP Goat anti-Mouse IgG (1: 5000, AS003, ABclonal).

Techniques: Migration, In Vitro, Transfection, Expressing

Journal: Journal of Translational Medicine

Article Title: Exosomes loaded with the anti-cancer molecule mir-1-3p inhibit intrapulmonary colonization and growth of human esophageal squamous carcinoma cells

doi: 10.1186/s12967-024-05997-9

Figure Lengend Snippet: miR-1-3p inhibits invasion of ESCC cells in vitro by down-regulating E2F5, which may involve the MAPK/ERK signaling pathway. ( A and B ) The invasion of KYSE150 and Eca109 cells in vitro co-transfected with miR-1-3p mimics and E2F5 expression plasmids. ( C ) The protein levels of p-p38, p38, p-ERK, ERK in transfected cells, detected with western blot. ns, no significance; *** P < 0.001

Article Snippet: Antibodies information used in this study is provided below: anti-TSG101 (1:2000, 14497-1-AP, Proteintech), anti-CD81 (66866-1-Ig), anti-CD9 (60232-1-Ig), anti-E2F5 (1:2000, bs-1734R, Bioss), anti-GAPDH (1:5000, 60004-1-Ig, Proteintech), anti-p-p38 (1:1000, 4511, CST), anti-p38 (1:5000, 66234-1-lg, Proteintech), anti-p-ERK (1:1000, AP0974, ABclonal), anti-ERK (1:700, A4782, ABclonal), anti-p-Akt (1:2000, 28731-1-AP, Proteintech), anti-p-mTOR (1:500, AF3308, Affinity), HRP Goat anti-Rabbit IgG (1: 5000, AS014, ABclonal), HRP Goat anti-Mouse IgG (1: 5000, AS003, ABclonal).

Techniques: In Vitro, Transfection, Expressing, Western Blot